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KMID : 0352720050290030145
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Journal of Ginseng Research
2005 Volume.29 No. 3 p.145 ~ p.151
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Effects of Addition of Inorganic Germanium, GeO₂ on the Growth, Germanium and Saponin Contents of Ginseng Adventitious Root in Submerged Culture
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장은정/Chang EJ
오훈일/Oh HI
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Abstract
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This study was carried out to determine the optimal submerged culture conditions for production of ginseng root containing germanium using plant tissue culture technology. The ginseng(Panax ginseng C.A. Meyer) root induced by plant growth regulators of 0.5 ㎎/L BAP and 3.0 ㎎/L NAA was cultured on SH medium and the effects of various GeO₂ concentrations, addition time of GeO₂ and pH of medium were investigated on fresh weight, saponin production and germanium accumulation in ginseng root. Optimal GeO₂ concentrations for fresh weight, saponin and germanium content were 10, 0 and 110ppm, respectively. When GeO₂ was added after 2 weeks cultivation of ginseng root, germanium content was higher than that of adding GeO₂ at the initial cultivation time, but saponin content and fresh weight were lower. pH 5.5 was found to be the most favorable condition for the growth of ginseng root and germanium accumulation, but saponin production was best at pH 6.0.
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